1. What is DNA fingerprinting? a) A method of identifying individuals based on their DNA sequences
    b) A method of creating identical copies of DNA
    c) A method of analyzing proteins in the body
    d) A method of determining the blood group of an individualAnswer: a) A method of identifying individuals based on their DNA sequences

  1. Which of the following is primarily used in DNA fingerprinting? a) Blood samples
    b) Hair samples
    c) DNA samples
    d) Urine samplesAnswer: c) DNA samples

  1. What is the primary application of DNA fingerprinting in forensics? a) Identifying genetic disorders
    b) Matching DNA found at a crime scene with suspects
    c) Producing recombinant DNA
    d) Analyzing food contaminationAnswer: b) Matching DNA found at a crime scene with suspects

  1. In DNA fingerprinting, what part of the DNA is analyzed? a) Exons
    b) Introns
    c) Non-coding regions (microsatellites)
    d) Mitochondrial DNA onlyAnswer: c) Non-coding regions (microsatellites)

  1. What technique is commonly used to analyze DNA for fingerprinting? a) Polymerase chain reaction (PCR)
    b) Gel electrophoresis
    c) Western blotting
    d) Both a and bAnswer: d) Both a and b

  1. Which of the following is true regarding the DNA samples used in forensic investigations? a) Only blood samples can be used
    b) DNA samples must come from living individuals
    c) DNA can be extracted from a variety of bodily fluids
    d) DNA cannot be extracted from hairAnswer: c) DNA can be extracted from a variety of bodily fluids

  1. What is the significance of STR (Short Tandem Repeats) in DNA fingerprinting? a) They provide a unique genetic fingerprint for individuals
    b) They determine the health of an individual
    c) They are responsible for gene expression
    d) They encode for proteins in the bodyAnswer: a) They provide a unique genetic fingerprint for individuals

  1. Which technique is used to amplify DNA in forensic DNA fingerprinting? a) Southern blotting
    b) Polymerase chain reaction (PCR)
    c) Electrophoresis
    d) DNA sequencingAnswer: b) Polymerase chain reaction (PCR)

  1. DNA fingerprinting can be used to identify: a) Paternity
    b) Criminal suspects
    c) Missing persons
    d) All of the aboveAnswer: d) All of the above

  1. Which of the following can provide DNA for fingerprinting? a) Hair with roots
    b) Semen
    c) Skin cells
    d) All of the aboveAnswer: d) All of the above

  1. In a forensic investigation, how is DNA evidence collected from a crime scene? a) By using a sample from a suspect’s mouth
    b) By swabbing areas that may contain biological fluids
    c) By collecting traces of fingerprints from a surface
    d) By extracting DNA from fingerprints left on a weaponAnswer: b) By swabbing areas that may contain biological fluids

  1. Which of the following is NOT a forensic application of DNA fingerprinting? a) Solving paternity disputes
    b) Identifying victims of disasters
    c) Creating genetically modified organisms
    d) Analyzing criminal casesAnswer: c) Creating genetically modified organisms

  1. What is the role of gel electrophoresis in DNA fingerprinting? a) To amplify DNA
    b) To separate DNA fragments based on size
    c) To extract DNA from cells
    d) To identify gene mutationsAnswer: b) To separate DNA fragments based on size

  1. Which of the following is a commonly used method for comparing DNA profiles in forensics? a) Comparison of mitochondrial DNA sequences
    b) RFLP (Restriction Fragment Length Polymorphism) analysis
    c) DNA sequencing
    d) KaryotypingAnswer: b) RFLP (Restriction Fragment Length Polymorphism) analysis

  1. What is the advantage of using DNA fingerprinting in forensic investigations? a) It is a faster method than other techniques
    b) It is highly accurate and can uniquely identify individuals
    c) It only works for certain types of crime
    d) It is not affected by the environmentAnswer: b) It is highly accurate and can uniquely identify individuals

  1. Which of the following are used to create a DNA profile? a) STR markers
    b) Single nucleotide polymorphisms (SNPs)
    c) Both a and b
    d) Only gene sequencesAnswer: c) Both a and b

  1. What is the role of DNA fingerprinting in identifying crime scene evidence? a) To compare unknown samples with known samples from suspects
    b) To find out the origin of the crime weapon
    c) To identify the motive behind the crime
    d) To identify the location of the crime sceneAnswer: a) To compare unknown samples with known samples from suspects

  1. Which of the following is true about the uniqueness of DNA fingerprinting? a) Each individual’s DNA is unique except for identical twins
    b) Only humans can be identified using DNA fingerprinting
    c) DNA profiles can be altered during an investigation
    d) All DNA samples are identical in a populationAnswer: a) Each individual’s DNA is unique except for identical twins

  1. What is a common challenge faced in DNA fingerprinting at crime scenes? a) Insufficient DNA samples available for analysis
    b) DNA contamination by other people at the scene
    c) Inability to store DNA samples long-term
    d) All of the aboveAnswer: d) All of the above

  1. What is the role of paternity testing in DNA fingerprinting? a) To determine the parental lineage of an individual
    b) To solve criminal investigations
    c) To identify rare genetic disorders
    d) To find inherited traitsAnswer: a) To determine the parental lineage of an individual

  1. How is DNA evidence typically stored for forensic analysis? a) In liquid nitrogen
    b) At room temperature with special care to prevent contamination
    c) In formaldehyde
    d) In blood serumAnswer: b) At room temperature with special care to prevent contamination

  1. What does STR stand for in the context of DNA fingerprinting? a) Short Target Region
    b) Short Tandem Repeat
    c) Standard Test Results
    d) Substantial Transfer ReactionsAnswer: b) Short Tandem Repeat

  1. What is the most commonly used DNA marker in forensic DNA fingerprinting? a) mtDNA (mitochondrial DNA)
    b) SNPs (Single Nucleotide Polymorphisms)
    c) STR (Short Tandem Repeats)
    d) RFLPs (Restriction Fragment Length Polymorphisms)Answer: c) STR (Short Tandem Repeats)

  1. What is the first step in the process of DNA fingerprinting? a) Gel electrophoresis
    b) PCR amplification of DNA
    c) Extracting DNA from biological samples
    d) Comparing DNA samples with databasesAnswer: c) Extracting DNA from biological samples

  1. Which of the following is a limitation of DNA fingerprinting? a) High cost of equipment
    b) Inability to provide conclusive evidence
    c) Long time to generate results
    d) Difficulty in amplifying DNA from small samplesAnswer: a) High cost of equipment

  1. What type of evidence can be used for DNA fingerprinting in forensic investigations? a) Blood
    b) Hair
    c) Semen
    d) All of the aboveAnswer: d) All of the above

  1. What is mitochondrial DNA used for in forensic DNA fingerprinting? a) To identify mutations in genes
    b) To provide maternal lineage information
    c) To create a complete DNA profile
    d) To study genetic disordersAnswer: b) To provide maternal lineage information

  1. How many loci are typically examined for creating a DNA profile in forensics? a) 10-15 loci
    b) 20-25 loci
    c) 35-45 loci
    d) 50-60 lociAnswer: a) 10-15 loci

  1. What is a major advantage of using DNA fingerprinting over other forensic techniques? a) It provides a visual representation of the suspect
    b) It is less time-consuming than other techniques
    c) It provides a highly accurate and unique identifier for individuals
    d) It can identify the criminal’s motiveAnswer: c) It provides a highly accurate and unique identifier for individuals

  1. What is the primary ethical concern related to the use of DNA fingerprinting in forensics? a) The accuracy of the technique
    b) Privacy and the potential misuse of genetic information
    c) Cost and availability of the technology
    d) The speed of DNA analysisAnswer: b) Privacy and the potential misuse of genetic information

These MCQs cover various aspects of DNA fingerprinting and its applications in forensic science, offering a comprehensive understanding of the subject.

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