1. What is the primary function of mitochondria in cellular respiration?
    Answer: Mitochondria produce ATP, the energy currency of the cell, through cellular respiration.
  2. Where does cellular respiration occur in eukaryotic cells?
    Answer: Cellular respiration occurs in the mitochondria.
  3. What are the two main stages of cellular respiration that take place in the mitochondria?
    Answer: The Krebs cycle and the electron transport chain.
  4. What is the role of NADH and FADH2 in mitochondrial respiration?
    Answer: They donate electrons to the electron transport chain to help produce ATP.
  5. What is the significance of the mitochondrial inner membrane?
    Answer: The inner membrane contains enzymes for the electron transport chain and ATP synthase, which are involved in ATP production.
  6. Where does the Krebs cycle occur within the mitochondria?
    Answer: The Krebs cycle occurs in the mitochondrial matrix.
  7. What is the function of the electron transport chain in mitochondria?
    Answer: It transfers electrons through protein complexes, releasing energy to pump protons across the membrane, creating a proton gradient for ATP synthesis.
  8. What is the final electron acceptor in the mitochondrial electron transport chain?
    Answer: Oxygen is the final electron acceptor, combining with electrons and protons to form water.
  9. What molecule is produced in the mitochondrial matrix during the Krebs cycle?
    Answer: ATP, NADH, FADH2, and carbon dioxide are produced during the Krebs cycle.
  10. What is the role of ATP synthase in the mitochondria?
    Answer: ATP synthase uses the proton gradient to synthesize ATP from ADP and inorganic phosphate.
  11. How does the proton gradient contribute to ATP production?
    Answer: The proton gradient across the inner membrane of the mitochondrion drives the production of ATP by ATP synthase.
  12. What molecule enters the mitochondria to begin the Krebs cycle?
    Answer: Acetyl-CoA enters the mitochondria and is used in the Krebs cycle.
  13. What is the source of energy that powers the electron transport chain in mitochondria?
    Answer: The energy comes from electrons carried by NADH and FADH2.
  14. What is the function of oxygen in cellular respiration?
    Answer: Oxygen serves as the final electron acceptor in the electron transport chain and helps produce water.
  15. What is the main byproduct of mitochondrial cellular respiration?
    Answer: The main byproducts are carbon dioxide and water.
  16. What role do the cristae of the mitochondria play in cellular respiration?
    Answer: The cristae increase the surface area for the electron transport chain and ATP synthesis.
  17. Which type of phosphorylation occurs in the mitochondria?
    Answer: Oxidative phosphorylation occurs in the mitochondria during the electron transport chain.
  18. What is the role of the mitochondrial matrix?
    Answer: The mitochondrial matrix is where the Krebs cycle takes place, and it contains enzymes, substrates, and cofactors for respiration.
  19. How many ATP molecules are typically produced from the complete oxidation of one glucose molecule in the mitochondria?
    Answer: Typically, 32 to 34 ATP molecules are produced.
  20. Which part of mitochondria contains its DNA and ribosomes?
    Answer: The mitochondrial matrix contains the DNA and ribosomes.
  21. What is produced during oxidative phosphorylation in mitochondria?
    Answer: ATP is produced during oxidative phosphorylation.
  22. How do NADH and FADH2 contribute to ATP production in mitochondria?
    Answer: NADH and FADH2 donate electrons to the electron transport chain, which drives proton pumping and ATP production.
  23. Where does glycolysis take place, and what role does it play in mitochondrial respiration?
    Answer: Glycolysis occurs in the cytoplasm and produces pyruvate, which is transported into the mitochondria for further processing.
  24. What is the role of the outer mitochondrial membrane?
    Answer: The outer membrane acts as a barrier and regulates the entry and exit of molecules into and out of the mitochondrion.
  25. What happens to the protons (H+) during the electron transport chain in mitochondria?
    Answer: Protons are pumped from the matrix to the intermembrane space, creating a proton gradient.
  26. How is ATP produced during the Krebs cycle?
    Answer: ATP is produced by substrate-level phosphorylation during the Krebs cycle.
  27. What molecule is required for the electron transport chain to function properly?
    Answer: Oxygen is required as the final electron acceptor.
  28. What is the significance of the mitochondrial DNA in cellular respiration?
    Answer: Mitochondrial DNA encodes some of the proteins needed for mitochondrial function, including those involved in ATP production.
  29. How does the ATP yield from anaerobic respiration compare to that of aerobic respiration in mitochondria?
    Answer: Aerobic respiration yields much more ATP than anaerobic respiration.
  30. What role does the mitochondrion play in energy metabolism beyond ATP production?
    Answer: Mitochondria are involved in other metabolic processes such as apoptosis, calcium storage, and regulation of the cell cycle.

These questions and answers provide a comprehensive overview of the role of mitochondria in cellular respiration, helping to clarify key concepts and functions.

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