1. Which enzyme is responsible for fixing carbon dioxide in the Calvin cycle?
    • a) RuBisCO
    • b) ATP synthase
    • c) Phosphofructokinase
    • d) Hexokinase
      Answer: a) RuBisCO
  2. What is the function of ATP synthase in cellular respiration?
    • a) Breaks down glucose
    • b) Synthesizes ATP from ADP and inorganic phosphate
    • c) Transport electrons
    • d) Fixes carbon dioxide
      Answer: b) Synthesizes ATP from ADP and inorganic phosphate
  3. Which enzyme catalyzes the first step of the Calvin cycle, the fixation of CO₂ to ribulose bisphosphate?
    • a) Phosphoglycerate kinase
    • b) Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO)
    • c) Malate dehydrogenase
    • d) Glyceraldehyde-3-phosphate dehydrogenase
      Answer: b) Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO)
  4. In glycolysis, which enzyme catalyzes the conversion of glucose to glucose-6-phosphate?
    • a) Phosphoglucose isomerase
    • b) Hexokinase
    • c) Phosphofructokinase
    • d) Aldolase
      Answer: b) Hexokinase
  5. Which enzyme is responsible for the production of NADPH in the light-dependent reactions of photosynthesis?
    • a) NADP+ reductase
    • b) ATP synthase
    • c) RuBisCO
    • d) Cytochrome c reductase
      Answer: a) NADP+ reductase
  6. Which enzyme catalyzes the reduction of 3-phosphoglycerate to glyceraldehyde-3-phosphate in the Calvin cycle?
    • a) Phosphoglycerate kinase
    • b) Glyceraldehyde-3-phosphate dehydrogenase
    • c) RuBisCO
    • d) Transketolase
      Answer: b) Glyceraldehyde-3-phosphate dehydrogenase
  7. Which enzyme facilitates the breakdown of glucose into two molecules of pyruvate during glycolysis?
    • a) Hexokinase
    • b) Phosphofructokinase
    • c) Aldolase
    • d) Pyruvate kinase
      Answer: c) Aldolase
  8. Which enzyme is responsible for converting pyruvate into acetyl-CoA in the mitochondria?
    • a) Pyruvate dehydrogenase
    • b) Hexokinase
    • c) Citrate synthase
    • d) Malate dehydrogenase
      Answer: a) Pyruvate dehydrogenase
  9. Which enzyme catalyzes the synthesis of ATP in the final step of oxidative phosphorylation?
    • a) ATP synthase
    • b) Cytochrome c oxidase
    • c) NADH dehydrogenase
    • d) Succinate dehydrogenase
      Answer: a) ATP synthase
  10. Which enzyme is involved in the fixation of atmospheric nitrogen in nitrogen-fixing bacteria during the process of nitrogen fixation?
    • a) Nitrogenase
    • b) RuBisCO
    • c) ATP synthase
    • d) Amylase
      Answer: a) Nitrogenase
  11. Which enzyme is involved in the synthesis of glucose from pyruvate during gluconeogenesis?
    • a) Hexokinase
    • b) Phosphoenolpyruvate carboxykinase
    • c) Pyruvate kinase
    • d) Aldolase
      Answer: b) Phosphoenolpyruvate carboxykinase
  12. Which enzyme in the citric acid cycle catalyzes the conversion of citrate to isocitrate?
    • a) Citrate synthase
    • b) Aconitase
    • c) Malate dehydrogenase
    • d) Succinate dehydrogenase
      Answer: b) Aconitase
  13. Which enzyme is crucial for the reduction of NADP+ to NADPH in the light-dependent reactions of photosynthesis?
    • a) NADP+ reductase
    • b) ATP synthase
    • c) Cytochrome c oxidase
    • d) Ribulose bisphosphate carboxylase
      Answer: a) NADP+ reductase
  14. Which enzyme helps in the production of acetyl-CoA from pyruvate in aerobic respiration?
    • a) Pyruvate dehydrogenase
    • b) Citrate synthase
    • c) Succinate dehydrogenase
    • d) Lactate dehydrogenase
      Answer: a) Pyruvate dehydrogenase
  15. Which enzyme is responsible for the conversion of ribulose-1,5-bisphosphate to 3-phosphoglycerate in the Calvin cycle?
    • a) RuBisCO
    • b) Phosphoglycerate kinase
    • c) Aldolase
    • d) Glyceraldehyde-3-phosphate dehydrogenase
      Answer: a) RuBisCO
  16. Which enzyme in the electron transport chain accepts electrons from NADH?
    • a) NADH dehydrogenase
    • b) Cytochrome c oxidase
    • c) Cytochrome b-c1 complex
    • d) ATP synthase
      Answer: a) NADH dehydrogenase
  17. Which enzyme is responsible for synthesizing ribulose-1,5-bisphosphate during the Calvin cycle?
    • a) Ribulose-5-phosphate kinase
    • b) RuBisCO
    • c) Glyceraldehyde-3-phosphate dehydrogenase
    • d) ATP synthase
      Answer: a) Ribulose-5-phosphate kinase
  18. Which enzyme converts phosphoenolpyruvate to oxaloacetate in the citric acid cycle?
    • a) Phosphoenolpyruvate carboxylase
    • b) Pyruvate kinase
    • c) Citrate synthase
    • d) Malate dehydrogenase
      Answer: a) Phosphoenolpyruvate carboxylase
  19. Which enzyme is involved in the regeneration of RuBP in the Calvin cycle?
    • a) Phosphoribulokinase
    • b) Glyceraldehyde-3-phosphate dehydrogenase
    • c) RuBisCO
    • d) Ribulose-5-phosphate isomerase
      Answer: a) Phosphoribulokinase
  20. Which enzyme catalyzes the final step in glycolysis, converting phosphoenolpyruvate to pyruvate?
    • a) Pyruvate kinase
    • b) Aldolase
    • c) Phosphofructokinase
    • d) Hexokinase
      Answer: a) Pyruvate kinase
  21. In photosynthesis, which enzyme is responsible for the production of oxygen from water molecules?
    • a) Oxygen-evolving complex (OEC)
    • b) ATP synthase
    • c) NADP+ reductase
    • d) Cytochrome b6f complex
      Answer: a) Oxygen-evolving complex (OEC)
  22. Which enzyme converts 3-phosphoglycerate to 1,3-bisphosphoglycerate in the Calvin cycle?
    • a) Glyceraldehyde-3-phosphate dehydrogenase
    • b) Phosphoglycerate kinase
    • c) RuBisCO
    • d) Transketolase
      Answer: b) Phosphoglycerate kinase
  23. What is the role of the enzyme NADH dehydrogenase in cellular respiration?
    • a) It transfers electrons from NADH to the electron transport chain
    • b) It synthesizes ATP
    • c) It converts glucose into pyruvate
    • d) It regulates the citric acid cycle
      Answer: a) It transfers electrons from NADH to the electron transport chain
  24. Which enzyme in the citric acid cycle converts succinate to fumarate?
    • a) Fumarase
    • b) Succinate dehydrogenase
    • c) Aconitase
    • d) Malate dehydrogenase
      Answer: b) Succinate dehydrogenase
  25. Which enzyme is responsible for the production of citric acid from acetyl-CoA and oxaloacetate?
    • a) Citrate synthase
    • b) Malate dehydrogenase
    • c) Pyruvate kinase
    • d) NADH dehydrogenase
      Answer: a) Citrate synthase
  26. Which enzyme catalyzes the decarboxylation of isocitrate to alpha-ketoglutarate in the citric acid cycle?
    • a) Isocitrate dehydrogenase
    • b) Citrate synthase
    • c) Aconitase
    • d) Malate dehydrogenase
      Answer: a) Isocitrate dehydrogenase
  27. Which enzyme is involved in the conversion of malate to oxaloacetate in the citric acid cycle?
    • a) Malate dehydrogenase
    • b) Aconitase
    • c) Succinate dehydrogenase
    • d) NADH dehydrogenase
      Answer: a) Malate dehydrogenase
  28. Which enzyme is responsible for reducing oxygen to water in the electron transport chain?
    • a) Cytochrome c oxidase
    • b) ATP synthase
    • c) NADH dehydrogenase
    • d) Cytochrome b-c1 complex
      Answer: a) Cytochrome c oxidase
  29. Which enzyme is responsible for catalyzing the splitting of water molecules in the light-dependent reactions of photosynthesis?
    • a) Water-splitting complex (Oxygen-evolving complex)
    • b) Cytochrome b6f complex
    • c) RuBisCO
    • d) NADP+ reductase
      Answer: a) Water-splitting complex (Oxygen-evolving complex)
  30. Which enzyme catalyzes the conversion of pyruvate into lactate in anaerobic conditions?
    • a) Lactate dehydrogenase
    • b) Pyruvate kinase
    • c) Phosphofructokinase
    • d) Hexokinase
      Answer: a) Lactate dehydrogenase

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