1. What is the central dogma of molecular biology?
    • A) DNA to RNA to Protein
    • B) Protein to RNA to DNA
    • C) RNA to DNA to Protein
    • D) DNA to Protein to RNA
    • Answer: A) DNA to RNA to Protein
  2. Which process converts DNA to RNA?
    • A) Translation
    • B) Transcription
    • C) Replication
    • D) Protein synthesis
    • Answer: B) Transcription
  3. What is the process of converting RNA into protein called?
    • A) Transcription
    • B) Replication
    • C) Translation
    • D) Splicing
    • Answer: C) Translation
  4. What molecule is synthesized during transcription?
    • A) mRNA
    • B) tRNA
    • C) rRNA
    • D) DNA
    • Answer: A) mRNA
  5. Where does transcription occur in eukaryotic cells?
    • A) Cytoplasm
    • B) Mitochondria
    • C) Nucleus
    • D) Ribosomes
    • Answer: C) Nucleus
  6. Which enzyme is responsible for transcription in cells?
    • A) RNA polymerase
    • B) DNA polymerase
    • C) Ligase
    • D) Helicase
    • Answer: A) RNA polymerase
  7. Which of the following is NOT involved in translation?
    • A) mRNA
    • B) tRNA
    • C) Ribosomes
    • D) DNA polymerase
    • Answer: D) DNA polymerase
  8. What is the primary function of ribosomes in translation?
    • A) To synthesize RNA
    • B) To assemble amino acids into proteins
    • C) To replicate DNA
    • D) To transcribe genes
    • Answer: B) To assemble amino acids into proteins
  9. What does tRNA carry during translation?
    • A) Amino acids
    • B) mRNA
    • C) DNA
    • D) Ribosomes
    • Answer: A) Amino acids
  10. Which type of RNA carries the genetic information from DNA to the ribosome?
    • A) rRNA
    • B) mRNA
    • C) tRNA
    • D) snRNA
    • Answer: B) mRNA
  11. What happens during the process of transcription?
    • A) DNA is replicated
    • B) RNA is synthesized from a DNA template
    • C) Proteins are made
    • D) tRNA is synthesized
    • Answer: B) RNA is synthesized from a DNA template
  12. What is the role of RNA polymerase in transcription?
    • A) It unzips the DNA double helix
    • B) It catalyzes the formation of mRNA from DNA
    • C) It binds to ribosomes
    • D) It synthesizes proteins
    • Answer: B) It catalyzes the formation of mRNA from DNA
  13. In eukaryotes, where does the mRNA undergo processing before it leaves the nucleus?
    • A) It is transcribed into rRNA
    • B) Introns are removed, and exons are spliced together
    • C) It is translated into protein
    • D) It is replicated
    • Answer: B) Introns are removed, and exons are spliced together
  14. Which sequence of bases in mRNA is responsible for specifying an amino acid?
    • A) Codon
    • B) Anticodon
    • C) Promoter
    • D) Terminator
    • Answer: A) Codon
  15. What is the function of the ribosome during translation?
    • A) To decode mRNA and synthesize proteins
    • B) To synthesize RNA from DNA
    • C) To replicate DNA
    • D) To transport amino acids
    • Answer: A) To decode mRNA and synthesize proteins
  16. In the process of translation, which molecule pairs with the mRNA codon?
    • A) Ribosome
    • B) tRNA
    • C) RNA polymerase
    • D) DNA
    • Answer: B) tRNA
  17. What happens to mRNA after translation is complete?
    • A) It is degraded
    • B) It is replicated
    • C) It becomes part of the ribosome
    • D) It enters the nucleus
    • Answer: A) It is degraded
  18. Which of the following is a correct sequence of events in protein synthesis?
    • A) Replication → Transcription → Translation
    • B) Transcription → Replication → Translation
    • C) Transcription → Translation → Replication
    • D) Translation → Transcription → Replication
    • Answer: C) Transcription → Translation → Replication
  19. What is the sequence of three bases in tRNA that is complementary to the mRNA codon called?
    • A) Exon
    • B) Codon
    • C) Anticodon
    • D) Promoter
    • Answer: C) Anticodon
  20. What is the purpose of the promoter region in DNA?
    • A) It signals the end of transcription
    • B) It binds to RNA polymerase to initiate transcription
    • C) It attaches to ribosomes for translation
    • D) It codes for amino acids
    • Answer: B) It binds to RNA polymerase to initiate transcription
  21. In prokaryotes, where does transcription occur?
    • A) Nucleus
    • B) Mitochondria
    • C) Cytoplasm
    • D) Ribosome
    • Answer: C) Cytoplasm
  22. Which of the following molecules is involved in both transcription and translation?
    • A) rRNA
    • B) DNA
    • C) mRNA
    • D) tRNA
    • Answer: C) mRNA
  23. What determines the sequence of amino acids in a protein?
    • A) The sequence of nucleotides in DNA
    • B) The sequence of nucleotides in RNA
    • C) The ribosome structure
    • D) The codons in mRNA
    • Answer: A) The sequence of nucleotides in DNA
  24. What is the function of the 5′ cap and poly-A tail in eukaryotic mRNA?
    • A) They help the mRNA bind to the ribosome and protect it from degradation
    • B) They assist in the transcription process
    • C) They help mRNA splice introns
    • D) They encode the amino acid sequence
    • Answer: A) They help the mRNA bind to the ribosome and protect it from degradation
  25. Which of the following is true about DNA replication and transcription?
    • A) Both processes produce RNA
    • B) Replication produces a DNA strand, while transcription produces RNA
    • C) Transcription requires DNA polymerase
    • D) Replication occurs in the ribosome
    • Answer: B) Replication produces a DNA strand, while transcription produces RNA
  26. What happens to the mRNA after it is transcribed in the nucleus?
    • A) It is translated into protein in the ribosome
    • B) It is spliced and processed before leaving the nucleus
    • C) It is stored in the nucleus
    • D) It is replicated
    • Answer: B) It is spliced and processed before leaving the nucleus
  27. In prokaryotic cells, what is the site of translation?
    • A) Nucleus
    • B) Cytoplasm
    • C) Mitochondria
    • D) Ribosome
    • Answer: B) Cytoplasm
  28. Which sequence in the DNA does RNA polymerase recognize to start transcription?
    • A) Exon
    • B) Introns
    • C) Promoter
    • D) Terminator
    • Answer: C) Promoter
  29. What is the main function of messenger RNA (mRNA)?
    • A) To bring amino acids to the ribosome
    • B) To carry genetic information from DNA to the ribosome
    • C) To form part of the ribosome
    • D) To catalyze protein synthesis
    • Answer: B) To carry genetic information from DNA to the ribosome
  30. What is the role of aminoacyl-tRNA synthetase in translation?
    • A) It catalyzes the binding of the tRNA to the mRNA
    • B) It adds the correct amino acid to the tRNA molecule
    • C) It assembles the ribosome
    • D) It initiates transcription
    • Answer: B) It adds the correct amino acid to the tRNA molecule

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